Sampling trabecular meshwork using TrabEx+

Abstract Background To report sampling of the trabecular meshwork using TrabEx+ (MicroSurgical Technology, Redmond, Washington, USA) device in ab interno trabeculectomy. Specifically, this series focusses upon preservation architecture for assessment glaucomatous features common histopathological techniques. Patients This six eyes undergoing with or without cataract surgery. Three patients had primary open angle glaucoma and remaining pigment dispersion glaucoma, ocular hypertension uveitic glaucoma. Four simultaneous Methods Trabecular was excised retrieved vitreoretinal forceps. The samples were then processed into formalin-fixed paraffin-embedded 4 micron tissue segments stained haematoxylin eosin, periodic acid–Schiff elastin Van Gieson. Collagen IV labelled immunohistochemistry purpose identifying basement membrane beams. Results Presence confirmed five taken. One consisted blood only, but expected following early termination procedure due to patient restlessness. In positive cases beams associated cells identified on hematoxylin-eosin staining. retained their lamellar structure. underlying evident three specimens, whilst two specimens insufficient size collagen labelling. Conclusions case illustrates that can be utilised successfully retrieve sufficient architectural perseveration enable laboratory analysis.